Alzheimers disease and granulocyte density diversity

作者：Jaremo Petter^*; Milovanovic Micha; Buller Caroline; Nilsson Staffan; Winblad Bengt

来源：European Journal of Clinical Investigation, 2013, 43(6): 545-548.

DOI：10.1111/eci.12072

• Alzheimers disease
• cell density
• eosinophils
• granulocytes
• neutrophils

Background The current study investigates circulating eosinophils and neutrophils in Alzheimers (AD) type dementia with respect to density (kg/L). The existence of -amyloid plaques in the brain is a feature of AD. Sporadic scientific reports indicate that the disease affects circulating neutrophils. In contrast, numerous publications investigate inflammatory reactions in AD brains. Locally, the plaques evoke a substantial inflammatory response involving activated microglia and astrocytes. Methods Subjects with probable AD (n=39) were included and compared with elderly individuals (n=22) lacking apparent memory problems. We sampled 10mL venous blood in citrate. Granulocytes were separated according to density in linear Percoll gradients. Subsequently, the gradients were divided into density subfractions (n=16). In every fraction, determination of eosinophil and neutrophil counts was carried out. Results AD sufferers displayed less granulocytes in fractions nos. 13-15 containing light cells. For these fractions, the P-values proved to be (P<0 center dot 001; not significant; P=0 center dot 03) and (P=0 center dot 01; P=0 center dot 01; not significant), for eosinophils and neutrophils, respectively. Conclusions The present work describes that less circulating light granulocytes are a feature of AD demented individuals. It is to hypothesize that it is a sign of impaired granulocyte turnover and cell damage. It is concluded that AD affects inflammatory cells in the periphery and that the behaviour of granulocytes in dementia is worthwhile further studies.

• 出版日期2013-6