Activation of Neutrophils by the Two-Component Leukotoxin LukE/D from Staphylococcus aureus: Proteomic Analysis of the Secretions

作者：Aslam Rizwan; Laventie Benoit Joseph; Marban Celine; Prevost Gilles; Keller Daniel; Strub Jean Marc; van Dorsselaer Alain; Haikel Youssef; Taddei Corinne; Metz Boutigue Marie Helene^*

来源：Journal of Proteome Research, 2013, 12(8): 3667-3678.

DOI：10.1021/pr400199x

• Staphylococcus aureus
• Leukotoxin LukE/D
• Neutrophils
• Chromogranin A/B
• Proteomic Analysis
• Antimicrobial Peptides
• Innate Immunity

Staphylococcus aureus is responsible for severe bacterial infections in hospitals and healthcare facilities. It produces single and bicomponent toxins (leukotoxins and hemolysins) that hinder innate immune function. Leukotoxin subunits bind to leukocyte cell membrane thus inducing transmembrane pores and subsequently, cell lysis. Leukotoxin LukE/D is a member of the bicomponent toxin family, but to date, no study concerning its involvement in host-pathogen interactions has been reported. In the present study, we performed the proteomic analysis of the secretions recovered after activation of human neutrophils by leukotoxin LukE/D. The neutrophil secretions were purified by RP-HPLC and different fractions were analyzed by Edman sequencing, LC-MS/MS, immunoblotted for chromogranin-derived peptides and further analyzed for antimicrobial properties. Proteomic analysis revealed that neutrophil secretions constitute a large number of proteins related with immune boosting mechanisms, proteolytic degradation, inflammatory process 2% and antioxidant reactions.

• 出版日期2013-8