Tomato SIRbohB, a member of the NADPH oxidase family, is required for disease resistance against Botrytis cinerea and tolerance to drought stress

作者：Li, Xiaohui; Zhang, Huijuan; Tian, Limei; Huang, Lei; Liu, Shixia; Li, Dayong^*; Song, Fengming

来源：Frontiers in Plant Science, 2015, 6: 463.

DOI：10.3389/fpls.2015.00463

• tomato (Solanum lycopersicum L.)
• respiratory burst oxidase homologs (Rbohs)
• Botlytis cinerea
• defense response
• drought stress tolerance

NADPH oxidases (also known as respiratory burst oxidase homologs, Rbohs) are key enzymes that catalyze the generation of reactive oxygen species (ROS) in plants. In the present study, eight SIRboh genes were identified in tomato and their possible involvement in resistance to Botrytis cinerea and drought tolerance was examined. Expression of ShRbohs was induced by B. cinerea and Pseudomonas syringae pv. tomato but displayed distinct patterns. Virus-induced gene silencing based silencing of SIFibohB resulted in reduced resistance to B. cinerea but silencing of other SIRbohs did not affect the resistance. Compared to non-silenced plants, the S/FibohB-silenced plants accumulated more ROS and displayed attenuated expression of defense genes after infection with B. cinerea. Silencing of SIFibohB also suppressed f1g22-induced ROS burst and the expression of SILrr22, a marker gene related to PAMP-triggered immunity (PTI). Transient expression of SIFibohB in Nicotiana benthamiana led to enhanced resistance to B. cinerea. Furthermore, silencing of SIFibohB resulted in decreased drought tolerance, accelerated water loss in leaves and the altered expression of drought-responsive genes. Our data demonstrate that SIRbohB positively regulates the resistance to B. cinerea, flg22-induced PTI, and drought tolerance in tomato.

• 出版日期2015-6-23
• 单位浙江大学