Analysis of variable sites between two complete South China tiger (Panthera tigris amoyensis) mitochondrial genomes

作者：Zhang Wenping; Yue Bisong^*; Wang Xiaofang; Zhang Xiuyue; Xie Zhong; Liu Nonglin; Fu Wenyuan; Yuan Yaohua; Chen Daqing; Fu Danghua; Zhao Bo; Yin Yuzhong; Yan Xiahui; Wang Xinjing; Zhang Rongying; Liu Jie; Li Maoping; Tang Yao; Hou Rong; Zhang Zhihe

来源：Molecular Biology Reports, 2011, 38(7): 4257-4264.

DOI：10.1007/s11033-010-0548-6

• Complete mitochondrial genome
• Panthera tigris amoyensis
• Variable site

In order to investigate the mitochondrial genome of Panthera tigris amoyensis, two South China tigers (P25 and P27) were analyzed following 15 cymt-specific primer sets. The entire mtDNA sequence was found to be 16,957 bp and 17,001 bp long for P25 and P27 respectively, and this difference in length between P25 and P27 occurred in the number of tandem repeats in the RS-3 segment of the control region. The structural characteristics of complete P. t. amoyensis mitochondrial genomes were also highly similar to those of P. uncia. Additionally, the rate of point mutation was only 0.3% and a total of 59 variable sites between P25 and P27 were found. Out of the 59 variable sites, 6 were located in 6 different tRNA genes, 6 in the 2 rRNA genes, 7 in non-coding regions (one located between tRNA-Asn and tRNA-Tyr and six in the D-loop), and 40 in 10 protein-coding genes. COI held the largest amount of variable sites (9 sites) and Cytb contained the highest variable rate (0.7%) in the complete sequences. Moreover, out of the 40 variable sites located in 10 protein-coding genes, 12 sites were nonsynonymous.

• 出版日期2011-10
• 单位成都大熊猫繁育研究基地; 四川大学; 福建梅花山华南虎繁育研究所