A dual-labeled Annexin A5 is not suited for SPECT imaging of brain cell death in experimental murine stroke

作者：Zille Marietta; Harhausenl Denise; De Saint Hubert Marijke; Michel Roger; Reutelingsperger Chris P; Dirnagl Ulrich^*; Wunder Andreas

来源：Journal of Cerebral Blood Flow and Metabolism, 2014, 34(9): 1568-1570.

DOI：10.1038/jcbfm.2014.115

• apoptosis
• cerebral ischemia
• mouse
• phosphatidylserine
• single-photon emission computed tomography

We previously showed that fluorescently labeled Annexin A5 (AnxA5); which binds specifically to phosphatidylserine on dead/dying cells, can be used in experimental stroke for monitoring cell death with optical imaging. However, optical imaging is not suitable for brain imaging in patients. Here we investigated whether a dual-labeled AnxA5 (technetium and fluorescence label) can be used for single-photon emission computed tomography (SPECT) of cell death in the same model. We detected dual-labeled AnxA5 in ethanol-induced cell death in the femur muscle, but not after middle cerebral artery occlusion (MCAO) at any time point, either with SPECT or with ex vivo autoradiography or fluorescence Microscopy. Dual-labeled AnxA5 appears to be unsuited for visualizing death of brain cells in this MCAO model.

• 出版日期2014-9