Analysis of antimicrobial resistance and class 1 integrons among strains from upper respiratory tract of healthy adults

作者：Liu, Haiyan; Wang, Hai; Huang, Mao; Mei, Yaning; Gu, Bing^*; Wu, Rui; Huang, Yiling; Chen, Yi; Xu, Yanling; Wang, Tong

来源：Journal of Thoracic Disease, 2013, 5(2): 149-155.

DOI：10.3978/j.issn.2072-1439.2013.03.09

• Antimicrobial resistance
• upper respiratory tract
• class 1 integron
• gene cassettes
• healthy adults

Objective: The distribution and characterization of integrons among opportunistic pathogens from nasopharynx of healthy adults. @@@ Methods: A total of 1,019 nasopharyngeal samples from healthy adults were collected; bacteria were identified by API system; antibiotic susceptibility were tested by K-B method; class 1,2 and 3 integrons were examined by degenerate primers of the genetic content of integrons were analyzed by PCR and DNA sequencing. @@@ Results: Out of the 1,019 cases, 743 (72.9%) opportunistic pathogens were isolated. The top five commons organism identified were Coagulase-negative staphylococcus (n=404), Staphylococcus aureus (n=109), Haemophilus influenzae (n=74), Streptococcus pneumoniae (n=49) and Klebsiella pneumoniae (n=32). Eight (25.0%) isolates of K. pneumoniae produced the ESBLs. The isolated rates of S. aureus and H. influenzae were decreased with aging. 6.3% (2/32) K. pneumoniae isolates and 16.7% (1/8) Proteus isolates carried class 1 integrons. Among intI1-positive strains, sequencing analysis revealed that one of the integron positive K. pneumoniae isolates harbored gene cassette aadA5-dfrA17. Proteus isolates harbored gene cassette aadA2-dhfrXII. @@@ Conclusions: The results stressed the need for continued surveillance of bacteria from the asymptomatic carriers.

• 出版日期2013-4
• 单位南京医科大学