A novel mitochondria-targeted fluorescent probe for imaging hydrazine in living cells, tissues and animals

作者：Kong, Xiuqi; Dong, Baoli; Wang, Chao; Zhang, Nan; Song, Wenhui; Lin, Weiying^*

来源：Journal of Photochemistry and Photobiology A: Chemistry , 2018, 356: 321-328.

DOI：10.1016/j.jphotochem.2018.01.009

• Mitochondria-targeted
• Red fluorescent probe
• Hydrazine
• Cells and tissues fluorescent imaging
• In vivo fluorescent imaging

The cytotoxicity of hydrazine (N2H4) is closely associated with mitochondria damages, and real-timely detecting mitochondrial N2H4 is of great importance for the in-depth study of the pathophysiological functions of N2H4 in living system. Although some of N2H4 fluorescent probes have been developed, the selective fluorescence imaging of mitochondrial N2H4 has rarely been reported. Herein, we present a novel mitochondria-targeted fluorescent probe (Rho-N2H4) for imaging N2H4 in living system. In the Rho-N2H4 system, a rhodamine derivative Rho with red emission was employed as the fluorophore, and acetyl group was utilized as response site for hydrazine. Upon treatment with N2H4, Rho-N2H4 displayed a drastically fluorescent signal at 645 nm. With the aid of Rho-N2H4, the fluorescence imaging of mitochondrial N2H4 in living cells was achieved. Assisted by high-definition 3D imaging, Rho-N2H4 can be applied for the imaging of N2H4 in living tissues with a penetration depth of about 50 mu m with red emission manner. Furthermore, in vivo experiments demonstrated that Rho-N2H4 can be capable of imaging N2H4 in living animals. We expect that Rho-N2H4 could act as a promising tool for in-depthly unveiling the physiological and pathological roles of mitochondrial N2H4 in living system.

• 出版日期2018-4-1
• 单位济南大学