DETERMINATION OF LANDIOLOL IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

作者：Aomori Tohru; Matsumoto Naoki; Nakamura Katsunori; Fujita Yukiyoshi; Sekizuka Masayuki; Araki Takuya; Nakamura Tomonori; Yamamoto Koujirou^*

来源：Journal of Liquid Chromatography & Related Technologies, 2011, 34(19): 2411-2419.

DOI：10.1080/10826076.2011.591022

• HPLC-fluorescence detector
• landiolol
• physostigmine
• propanolol
• pseudocholinesterase
• rapid quantification method

Landiolol is an ultra-short-acting beta(1)-adrenergic receptor-blocking agent that is used with perioperative patients who are at risk of cardiac events. The drug is hydrolyzed very rapidly by pseudocholinesterase in the blood. Using physostigmine (final concentration: 10(-4) mol/L) as a cholinesterase inhibitor, we developed a simple and rapid quantification method for landiolol using high-performance liquid chromatography. Chromatographic separations were achieved on a C(18) column with 20 mM KH(2)PO(4)/acetonitrile (70/30, v/v). Propranolol was used as an internal standard. The peaks were detected using a fluorescence detector (excitation 220 nm and emission 310 nm). The retention times of landiolol and propranolol were 5.6 min and 6.7 min, respectively. The lower limit of quantification was estimated as being 0.1 mu g/mL. The precision of the inter-and intra-assay varied between 1.8% and 12.6%, and the accuracy varied between -8.1% and 7.7%. This method was useful for the determination of landiolol in human plasma after intravenous administration.

• 出版日期2011