Molecular cloning and characterization of a cDNA encoding a novel antibacterial peptide, defensin, from the mulberry longicorn beetle, Apriona germari

作者：Lu Wei; Cui Zheng; Wang Dong; Lee Kwang Sik; Park Nam Sook; Kim Doh Hoon^*; Jin Byung Rae

来源：DNA Sequence, 2007, 18(2): 160-164.

DOI：10.1080/10425170601108639

• antibacterial peptide
• Apriona germari
• cDNA
• cysteine-rich peptide
• defensin
• mulberry longicorn beetle

A full-length cDNA clone with high homology ( 62% mature peptide sequence identity) to an Acalolepta luxuriosa antibacterial gene, possessing a conserved cysteine-stabilized ab motif, was cloned by screening an Apriona germari cDNA library. This gene ( AgCRP) had a total length of 360 bp with an open reading frame of 207 bp, and encoded a predicted peptide of 69 amino acid residues. The mature AgCRP peptide was 27 amino acid residues long and had a cysteine-stabilized ab motif of C...CXXXC...C...CXC consensus sequence, similar to insect defensins. Northern blot analysis revealed that the AgCRP exhibited fat body-specific expression and was up-regulated by wounding, bacterial or fungal challenge.

• 出版日期2007
• 单位沈阳药科大学