ASSESSING THE DIVERSITY OF BACTERIAL COMMUNITIES ASSOCIATED WITH PLANTS

作者：Andreote Fernando Dini^*; Azevedo Joao Lucio; Araujo Welington Luiz

来源：Brazilian Journal of Microbiology, 2009, 40(3): 417-432.

DOI：10.1590/s1517-83822009000300001

• Plant-bacteria interactions
• molecular techniques
• multivariate analysis
• endophytes
• rhizosphere

Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information about the development of traditional methods and a general overview about the new insights into bacterial communities associated with plants.

• 出版日期2009-9