Altering substrate specificity of catechol 2,3-dioxygenase from Planococcus sp strain S5 by random mutagenesis

作者：Hupert Kocurek Katarzyna^*; Wojcieszynska Danuta; Guzik Urszula

来源：Acta Biochimica Polonica, 2014, 61(4): 705-710.

• Planococcus
• catechol 2,3-dioxygenase
• substrate specificity
• mutagenesis

c23o gene, encoding catechol 2,3-dioxygenase from Planococcus sp. strain S5 was randomly mutagenized to generate variant forms of the enzyme with higher degradation activity. Additionally, the effect of introduced mutations on the enzyme structure was analyzed based on the putative 3D models the wild-type and mutant enzymes. C23OB58 and C23OB81 mutant proteins with amino acid substitutions in close proximity to the enzyme surface or at the interface and in the vicinity of the enzyme active site respectively showed the lowest activity towards all catecholic substrates. The relative activity of C23OC61 mutant towards para-substituted catechols was 20-30% lower of the wild-type enzyme. In this mutant all changes: F19l, C268R, Y272H, V280A and Y293D were located within the conserved regions of C-terminal domain. From these F191l seems to have significant implications for enzyme activity. The highest activity towards different catechols was found for mutant C23OB65. R296Q mutation improved the activity of C230 especially against 4-chlorocatechol. The relative activity of above-mentioned mutant detected against this substrate was almost 6-fold higher than the wildtype enzyme. These results should facilitate future engineering of the enzyme for bioremediation.

• 出版日期2014