Astakine LvAST binds to the beta subunit of F-1-ATP synthase and likely plays a role in white shrimp Litopeneaus vannamei defense against white spot syndrome virus

作者：Liang, Gao-Feng; Liang, Yan; Xue, Qinggang^*; Lu, Jin-Feng; Cheng, Jun-Jun; Huang, Jie

来源：Fish & Shellfish Immunology, 2015, 43(1): 75-81.

DOI：10.1016/j.fsi.2014.12.015

• Astakine
• Invertebrate cytokine
• White spot syndrome virus
• Shrimp antiviral immunity
• F-1-ATP synthase beta subunit

Cytokines play a critical role in innate and adaptive immunity. Astakines represent a group of invertebrate cytokines that are related to vertebrate prokineticin and function in promoting hematopoiesis in crustaceans. We have identified an astakine from the white shrimp Litopeneaus vannamei and named it LvAST in a previous research. In the present research, we investigated the interactions among LvAST, the envelope protein VP37 of white spot syndrome virus (i.e., WSSV), and the beta subunit of F-1-ATP synthase (ATPsyn-beta) of the white shrimp (i.e., BP53) using binding assays and co-precipitations. We also examined the effects of LvAST on shrimp susceptibility to WSSV. We found that LvAST and VP37 competitively bound to BP53, but did not bind to each other. Shrimps that had been injected with recombinant LvAST exhibited significantly lower mortality and longer survival time in experimental infections by WSSV. In contrast, shrimps whose LvAST gene expression had been inhibited by RNA interference showed significantly higher WSSV infection intensity and shorter survival time following viral challenges. These results suggested that LvAST and WSSV both likely use ATPsyn-beta as a receptor and LvAST plays a role in shrimp defense against WSSV infection. This represented the first research showing the involvement of astakines in host antiviral immunity.

• 出版日期2015-3
• 单位中国水产科学研究院