An Easy-to-Use Polystyrene Microchip-based Cell Culture System

作者：Tazawa Hidekatsu^*; Sunaoshi Shohei; Tokeshi Manabu; Kitamori Takehiko; Ohtani Kaneko Ritsuko

来源：Analytical Sciences, 2016, 32(3): 349-353.

DOI：10.2116/analsci.32.349

• Microchip
• microfluidics
• cell assay
• bubble trap
• endothelial cell
• cell-response monitoring

In this study, we developed an integrated, low-cost microfluidic cell culture system that is easy to use. This system consists of a disposable polystyrene microchip, a polytetrafluoroethylene valve, an air bubble trap, and an indium tin oxide temperature controller. Valve pressure resistance was validated with a manometer to be 3 MPa. The trap protected against bubble contamination. The temperature controller enabled the culture of Macaca mulatta RF/6A 135 vascular endothelial cells, which are difficult to culture in glass microchips, without a CO2 incubator. We determined the optimal coating conditions for these cells and were able to achieve stable, confluent culture within 1 week. This practical system is suitable for low-cost screening and has potential applications as circulatory cell culture systems and research platforms in cell biology.

• 出版日期2016-3