Astragaloside IV inhibits hypoxia-induced cardiac myocytes death via PKC beta/Egr-1 pathway

作者：Zhang, Ying; Li, Hong; Zhang, Qin; Lou, Xiao-Ming; Chen, Guo-Qing^*

来源：Biomedical Research-India, 2017, 28(11): 4774-4777.

• Astragaloside IV
• Hypoxia/reoxygenation injury
• PKC beta
• Egr-1
• Neonatal rat Cardiac myocytes

Astragaloside IV exerts beneficial effects on hypoxia/reoxygenation-induced cardiomyocyte injury. However, the exact mechanisms needs further disclosed. This study was designed to investigate the role of PKC beta/Egr-1 pathway in the protective effect of Astragaloside IV on hypoxia/reoxygenation injury. Exposed under hypoxia/reoxygenation condition, the primary cultured neonatal rat cardiac myocytes viability was reduced significantly. While the expression of Egr-1, the phosphorylated level of PKC beta and ERK kinase, and the activities of SOD and MDA were up-regulated obviously. On the other hand, the cells with Astragaloside IV preconditioning increased cell survival rate compared with hypoxia/reoxygenation alone. Moreover, the expression of Egr-1, the phosphorylated level of PKC beta, and ERK was decreased in Astragaloside IV group. Taken together, these results suggest that the cardioprotection of Astragaloside IV may be through the down-regulation of PKC beta/Egr-1 pathway.

• 出版日期2017
• 单位浙江省人民医院