Animal and human enteric viruses in water and sediment samples from dairy farms

作者：Staggemeier Rodrigo^*; Bortoluzzi Marina; da Silva Heck Tatiana Moraes; da Luz Roger Bordin; Fabres Rafael Bandeira; Soliman Mayra Cristina; Rigotto Caroline; Baldasso Nelson Antonio; Spilki Fernando Rosado; de Matos Almeida Sabrina Esteves

来源：Agricultural Water Management, 2015, 152: 135-141.

DOI：10.1016/j.agwat.2015.01.010

• Infectious virus
• Dairy farms
• ICC-RT-qPCR

The detection of enteric viruses accompanied by a characterization of the viruses found in a given environmental matrix may inform about the sources of fecal contamination. In the present work, 55 water samples and 20 sediment samples were collected from 21 small farms in southern Brazil. Coliform counting was done as well as molecular detection of human enterovirus (EV), and human and animal adenovirus es. Viral detection was performed using real-time quantitative PCR (qPCR). Furthermore, the viral viability of human AdV (HAdV) by ICC-RT-qPCR in sediment and water samples was analyzed. Regarding to the coliforms, only 72.7% of the samples showed fecal contamination. HAdV was detected in 87.3% of water samples, followed by AvAdV (27.3%), CAV (20%), BAV (7.3%) and PoAdV or EV (1.8%). From the sediment samples, HAdV (80%) followed by CAV (20%), BAV (5%) and no positive results for PoAdV or EV. The viral loads ranged from 1.57 x 10(2) gc/L up to 6.68 x 10(9) gc/L (water), and from 1.97 x 10(3) gc/g to 2.18 x 10(8) gc/g (sediment). Most of these viral particles in water should be non-infectious, since after the ICC-PCR, HAdV was detected in only 4 samples (8.8%). On the other hand, it is noticeable that 5 sediment samples (25%) gave positive results for the presence of infectious viral particles.

• 出版日期2015-4