Defining Blood Processing Parameters for Optimal Detection of gamma-H2AX Foci: A Small Blood Volume Method

作者：Wojewodzka Maria; Sommer Sylwester; Kruszewski Marcin; Sikorska Katarzyna; Lewicki Maciej; Lisowska Halina; Wegierek Ciuk Aneta; Kowalska Magdalena; Lankoff Anna^*

来源：Radiation Research, 2015, 184(1): 95-104.

DOI：10.1667/RR13897.1

Biodosimetric methods used to measure the effects of radiation are critical for estimating the health risks to irradiated individuals or populations. The direct measurement of radiation-induced gamma-H2AX foci in peripheral blood lymphocytes is one approach that provides a useful end point for triage. Despite the documented advantages of the gamma-H2AX assay, there is considerable variation among laboratories regarding foci formation in the same exposure conditions and cell lines. Taking this into account, the goal of our study was to evaluate the influence of different blood processing parameters on the frequency of gamma-H2AX foci and optimize a small blood volume protocol for the gamma-H2AX assay, which simulates the finger prick blood collection method. We found that the type of fixative, temperature and blood processing time markedly affect the results of the gamma-H2AX assay. In addition, we propose a protocol for the gamma-H2AX assay that may serve as a potential guideline in the event of large-scale radiation incidents.

• 出版日期2015-7