A New Caffeine Test for Diagnosis of Cirrhosis by GC/MS

作者：Mesaros C^*; Culea M; Iordache A M; Visovan I; Cozar C; Cosma C

来源：Asian Journal of Chemistry, 2010, 22(5): 3608-3614.

• Caffeine test
• GC-MS
• Cirrhosis
• Clearance

A method for caffeine levels determination in children suffering from liver diseases by gas chromatography-mass spectrometry (GC/MS) is presented The selected ion monitoring (SIM) mode was used in caffeine test Measurements by adding known amounts of the (15)N labelled internal standard. The method was validated in the range 0-20 mu g/mL caffeine A single dose of 4 mg/kg p o of caffeine was followed by blood concentrations measurements at two points. 1 and 9 h Caffeine clearance, 0.74 +/- 049 mL min(-1) kg(-1), measured in patients with cirrhosis was reduced and half live time. t(1/2) = 14 73 +/- 12 36 h, was increased as compared with controls of 1 28 +/- 031 mL min(-1) kg(-1) and t(1/2) = 5.73 +/- 1 58 h

• 出版日期2010-5