Novel functionalized nanomaterials for the effective enrichment of proteins and peptides with post-translational modifications

作者：Nunez Cristina; del Pilar Chantada Vazquez Maria; Bravo Susana B; Vazquez Estevez Sergio

来源：Journal of Proteomics, 2018, 181: 170-189.

DOI：10.1016/j.jprot.2018.04.017

• Sample pretreatment
• enrichment
• nanomaterial
• low-abundance proteins/peptides
• phosphoproteins/phosphopetides
• glycoproteins/glycopeptides

Low abundance proteins/peptides usually suffer strong interference with highly abundant proteins/peptides as well as other contaminants, resulting in low ionization efficiency in MS analysis. Hence, the enrichment and separation of proteins/peptides from complex mixtures are of great value to their successful identification.
In this review we present an overview of the application of different nanocomposites for the specific enrichment of peptides/proteins with post-translational modifications (PTMs), such as phosphorylation and glycosylation. Furthermore, examples of the selective enrichment of cysteine-containing peptides, histidine-tagged proteins and the isolation of N-terminal peptides will be analyzed.

• 出版日期2018-6-15