A comparison of non-integrating reprogramming methods

作者：Schlaeger, Thorsten M.^*; Daheron, Laurence; Brickler, Thomas R.; Entwisle, Samuel; Chan, Karrie; Cianci, Amelia; DeVine, Alexander; Ettenger, Andrew; Fitzgerald, Kelly; Godfrey, Michelle; Gupta, Dipti; McPherson, Jade; Malwadkar, Prerana; Gupta, Manav; Bell, Blair; Doi, Akiko; Jung, Namyoung; Li, Xin; Lynes, Maureen S.; Brookes, Emily; Cherry, Anne B. C.; Demirbas, Didem; Tsankov, Alexander M.; Zon, Leonard I.; Rubin, Lee L.; Feinberg, Andrew P.; Meissner, Alexander; Cowan, Chad A.

来源：Nature Biotechnology, 2015, 33(1): 58-U230.

DOI：10.1038/nbt.3070

• PLURIPOTENT STEM-CELLS
• HUMAN SOMATIC-CELLS
• HUMAN FIBROBLASTS
• IPS CELLS
• GENERATION
• DIFFERENTIATION
• DERIVATION
• INDUCTION
• VECTOR
• MOUSE

Human induced pluripotent stem cells (hiPSCs(1-3)) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)(4), episomal (Epi)(5) and mRNA transfection mRNA(6) methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.

• 出版日期2015-1