Aspirin Inhibits LPS-Induced Expression of PI3K/Akt, ERK, NF-kappa B, CX3CL1, and MMPs in Human Bronchial Epithelial Cells

作者：Jiang, Ronglin; Wei, Liling; Zhu, Meifei; Wu, Jiannong; Wang, Lingcong^*

来源：Inflammation, 2016, 39(2): 643-650.

DOI：10.1007/s10753-015-0289-8

• aspirin
• pI3K/Akt
• NF-kappa B
• CX3CL1
• MMP

This study focused on the effects of aspirin on lipopolysaccharide (LPS)-induced expression of phosphoinositide 3 kinase (PI3K)/protein kinase B (Akt), extracellular signal-regulated protein kinase (ERK), nuclear factor-kappa B (NF-kappa B), CX3CL1, and MMPs in human bronchial epithelial cells. Human bronchial epithelial cells were seeded in six-well plates. After 24 h, the cells were classified into six groups: control blank (CK) group; LPS group; PD98059 (ERK inhibitor) (PD) group, treated with LPS + ERK inhibitor; LY294002(PI3K/Akt inhibitor) (LY) group, treated with LPS + PI3K/Akt inhibitor; Aspirin (Asp) group, treated with LPS + aspirin; and Pyrrolidinedithiocarbamic acid (PDTC) group, treated with LPS + NF-kappa B inhibitor. After 4-h treatment, the cells were harvested. Western blot analysis was performed to detect the expression of PI3K/Akt, ERK, NF-kappa B, and CX3CL1. Real-time quantitative PCR (RT-qPCR) was used to determine the gene expression of MMP-7, MMP-9, and MMP-12. Compared to the CK group, expression of PI3K/Akt, ERK, NF-kappa B, and CX3CL1 was significantly increased in the LPS group (P < 0.05). When compared to the LPS group, expression of PI3K/Akt, ERK, NF-kappa B, and CX3CL1 was significantly decreased in the PD group, PDTC group, and Asp group (P < 0.05). In addition, expression of NF-kappa B in the LY group was significantly reduced by comparison with the LPS group (P < 0.05). RT-qPCR: When compared to the LPS group, expression of MMP-7 and MMP-12 was significantly decreased in Asp group (P < 0.05). Expression of MMP-12 was significantly reduced in LY group (P < 0.05). LPS-ERK, NF-kappa B-PI3K/Akt, and CX3CL1 signal pathways exist in human bronchial epithelial cells. The PI3K/Akt inhibitor repressed expression of MMP-12. Aspirin inhibited LPS-induced expression of PI3K, Akt, ERK, NF-kappa B, CX3CL1, MMP-7, and MMP-12 in human bronchial epithelial cells.

• 出版日期2016-4
• 单位浙江中医药大学