Auditory cells produce nitric oxide in response to bacterial lipopolysaccharide

作者：Tanigawa Tohru; Morikawa Akiko; Hayashi Ken; Dan Katsuaki; Tsuchihashi Nana; Goto Fumiyuki; Ueda Hiromi; Yokochi Takashi^*

来源：Innate Immunity, 2013, 19(2): 115-120.

DOI：10.1177/1753425912450347

• Auditory cells
• lipopolysaccharide
• nitric oxide
• Toll-like receptor
• HEI-OC1 cells

The NO productivity of auditory cells in response to LPS was examined by using conditionally immortalized murine HEI-OC1 auditory cells. HEI-OC1 cells produced NO in response to LPS ranging from 0.1 mu g/ml to 100 mu g/ml in a concentration-dependent manner. LPS at 100 mu g/ml exhibited no cytotoxic action against HEI-OC1 cells and led to the highest level of NO production. The NO output in LPS-treated HEI-OC1 cells gradually increased up to 72 h. LPS-induced NO production was mediated by the expression of an inducible NO synthase (iNOS) protein. TLR4 and CD14 was expressed on the cell surface of HEI-OC1 cells. LPS augmented the production of IFN-beta in the MyD88-independent pathway of LPS signalling. HEI-OC1 cells produced NO in response to a TLR2 ligand but not TLR3 ligand. LPS was suggested to lead to NO production in auditory cells via iNOS expression. The immunological significance of NO production in auditory cells is discussed.

• 出版日期2013-4