DETERMINATION OF PICOMOLE CONCENTRATIONS OF ALUMINUM (III) IN HUMAN SALIVA AND URINE BY A LUMINOL-CARBOXYMETHYL CHITOSAN CHEMILUMINESCENCE SYSTEM

作者：You, Judong; Song, Zhenghua^*

来源：Instrumentation Science & Technology, 2013, 41(5): 524-534.

DOI：10.1080/10739149.2013.796561

• Al(III)
• carboxymethyl chitosan
• chemiluminescence
• flow injection
• luminol

A luminol-carboxymethyl chitosan (CMCS) system was established using flow injection chemiluminescence (CL) based on the enhancing effect of CMCS on the luminol-dissolved oxygen reaction. The CL intensity was linear with the CMCS concentration ranging from 0.01-30.0 mu M. Al(III) was shown to quench the CL of the luminol-CMCS reaction, and the decrease of CL intensity was linear with the logarithm of Al(III) concentration over the range from 10-1000 pM with a detection limit of 3.5 pM (3 sigma). At a flow rate of 2.0mLmin(-1), the analysis was performed within 30s. The proposed CL method was successfully applied to the determination of picomole levels Al(III) in human saliva and urine after oral intake of two aluminum hydroxide tablets, with recoveries from 90.6-108.7% and relative standard deviations <3% (n=5). The results indicated that the excreted Al(III) in saliva and urine reached its maximum values at 3hr and 2hr, and the total excretive ratio were 1.24x10(-3)% and 3.45% in 6hr and 12hr, respectively. The elimination rate constant k and the half-life time t(1/2) in human saliva and urine were 0.3747hr(-1), 1.8495hr, 0.7132hr(-1), and 0.9717hr, respectively. The possible CL mechanism of the luminol-CMCS-Al(III) reaction is discussed.

• 出版日期2013-9-3
• 单位西北大学