Detection of Mycobacterium avium Subspecies paratuberculosis in Milk and fecal Samples in Dairy Cattle by PCR and Nested-PCR

作者：Moghadam M Tohidi^*; Sarv S; Moosakhani F; Badiie A

来源：Journal of Animal and Veterinary Advances, 2010, 9(24): 3055-3061.

• Cattle
• Mycobacterium avium
• subsp paratuberculosis
• single PCR
• nested-PCR
• milk
• feces

Mycobacterium avium ssp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in cattle. The disease causes diarrhea, reduced milk production, poor reproductively, infertility and emaciation. MAP was isolated from intestinal tissue of a human patient suffering from Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Milk may be a potential vehicle for the transmission of MAP to human population. In this study, milk and fecal samples were collected from cows (n = 150) with suspected of Johne's status. Milk and fecal samples were processed for Nested PCR, Nested PCR detected positive samples in milk and feces 68.150(45.3%) and 74.150 (49.3%), respectively and PCR detected positive samples in milk and feces 41.150 (27.3%) and 48.150 (32%), respectively.

• 出版日期2010