Antiplatelet activity of caffeic acid phenethyl ester is mediated through a cyclic GMP-dependent pathway in human platelets

作者：Tyng Guey Chen; Jie Jen Lee; Kuang Hung Lin; Chia Hung Shen; Duen Suey Chou; Joen Rong Sheu^*

来源：Chinese Journal of Physiology, 2007, 50(3): 121-126.

• caffeic acid phenethyl ester
• cyclic GMP
• vasodilator-stimulated phosphoprotein
• protein
• kinase C

The aim of this study was to examine the inhibitory mechanisms of caffeic acid phenethyl ester (CAPE), which is derived from the propolis of honeybee, in platelet activation. In this study, CAPE (15 and 25 mu M) markedly inhibited platelet aggregation stimulated by collagen (2 mu g/ml). CAPE (15 and 25 mu M) increased cyclic GMP level, and cyclic GMP-induced vasodilator-stimulated phosphoprotein (VASP) Ser(157) phosphorylation, but did not increase cyclic AMP in washed human platelets. Rapid phosphorylation of a platelet protein of Mw. 47,000 (P47), a marker of protein kinase C activation, was triggered by phorbol-12, 13-dibutyrate (150 nM). This phosphorylation was markedly inhibited by CAPE (15 and 25 gM). The present study reports a novel and potent antiplatelet agent, CAPE, which involved in the following inhibitory pathways: CAPE increases cyclic GMP/VASP Ser(157) phosphorylation, and subsequently inhibits protein kinase C activity, resulting in inhibition of P47 phosphorylation, which ultimately inhibits platelet aggregation. These results strongly indicate that CAPE appears to represent a novel and potent antiplatelet agent for treatment of arterial thromboembolism.

• 出版日期2007-6-30
• 单位河北医科大学