Analytical determination of flavonoids aimed to analysis of natural samples and active packaging applications

作者：del Mar Castro Lopez Maria; Manuel Lopez Vilarino Jose; Victoria Gonzalez Rodriguez Maria

来源：Food Chemistry, 2014, 150: 119-127.

DOI：10.1016/j.foodchem.2013.10.156

• Natural antioxidants
• Catechins
• Quercetin
• Chromatography
• Comparison
• Active packaging

Several HPLC and UHPLC developed methods were compared to analyse the natural antioxidants catechins and quercetin used in active packaging and functional foods. Photodiode array detector coupled with a fluorescence detector and compared with LTQ-Orbitrap-MS was used. UHPLC was investigated as quick alternative without compromising the separation, analysis time shortened up to 6-fold. The feasibility of the four developed methods was compared. Linearity up to 0.9995, low detection limits (between 0.02 and 0.7 for HPLC-PDA, 2 to 7-fold lower for HPLC- LTQ-Orbitrap-MS and from 0.2 to 2 mg L-1 for UHPLC-PDA) and good precision parameters (RSD lower than 0.06%) were obtained. All methods were successfully applied to natural samples. LTQ-Orbitrap-MS allowed to identify other analytes of interest too. Good feasibility of the methods was also concluded from the analysis of catechin and quercetin release from new active packaging materials based on polypropylene added with catechins and green tea.

• 出版日期2014-5-1
• 单位常州工学院