A SIMPLE APPROACH TO ENHANCE DETECTION IN CAPILLARY ELECTROPHORESIS

作者：Shihabi Zak K^*

来源：Journal of Liquid Chromatography & Related Technologies, 2010, 33(17): 1562-1575.

DOI：10.1080/10826076.2010.503756

• capillary electrophoresis
• detection
• mycophenolic acid
• procaineamide
• stacking
• wide capillaries

In spite of the fact that it offers several advantages, capillary electrophoresis (CE) continues to lag behind HPLC in routine use, mainly due to poor sensitivity of detection. In this paper, the sensitivity of detection is greatly improved through the combination of several simple steps: longer (50-100cm, long), and wider (75-100m, i.d.) capillaries to hold extra sample volume and improve both the signal to noise ratio and provide better separation. Simultaneously, the analysis is performed under general stacking methods in order to decrease the band width. In order to utilize the wider capillaries, the separation was also performed with special buffers, which generate low current: zwitterionic, low ionic strength, or with added ethylene glycol. Based on these modifications, compounds such as procaineamide and mycophenolic acid were analyzed with detection limits in par with the HPLC, but with much better plate numbers and at much less operating costs. Furthermore, this approach was extended to the analysis of the uremic toxin indoxyl sulfate in serum, which represents a difficult analysis in a very complex matrix.

• 出版日期2010