The influence of juvenile hormone analogue, fenoxycarb on the midgut remodeling in Bombyx mori (L., 1758) (Lepidoptera: Bombycidae) during larval-pupal metamorphosis

作者：Goncu Ebru^*; Parlak Osman

来源：Turkish Journal of Entomology, 2011, 35(2): 179-194.

• Apoptosis
• autophagic cell death
• Bombyx mori
• fenoxycarb
• midgut

During larval-pupal metamorphosis, midgut tissues of holometabolous insects undergo remodeling and this remodeling process involves two metamorphic events. Degeneration of larval midgut occurs via programmed cell death and pupal midgut develops from regenerative (stem) cells. All metamorphic events in insects are regulated by ecdysone and juvenile hormone. Molecular mechanisms of ecdysone are well known but action mechanism of juvenile hormone is not well understood. Fenoxycarb, 0-ethyl N-(2-(4-pheoxyphenoxy)-ethyl) carbamate has been previously shown one of the most potent juvenile hormone analogue against a variety of insect species and widely used for investigations the mechanisms of juvenile hormone. In this study, we aimed to analyze the metamorphic events in the midgut of Bombyx mori Linnaeus, 1758 and investigate the effects of fenoxycarb on this period. For this purpose, we applied two different doses of fenoxycarb (1ng/10 mu l and 10ng/10 mu l) topically on day 0 of fifth instar larvae. We followed the progression of remodeling processes with morphological observations and analyzed programmed cell death specific proteins, caspase 3 for apoptosis and acid phosphatase for autophagic cell death in control and experiment groups. Our results indicated that, programmed cell death of larval midgut has morphologic characteristics of apoptosis and caspase activation occurs during this period and juvenile hormone analogue fenoxycarb inhibits or delays remodeling process in dose dependent manner.

• 出版日期2011