Analysis of a second-generation tetracycline antibiotic minocycline in human plasma by LC-MS/MS

作者：Patel Dinesh S; Sharma Naveen; Patel Mukesh C; Patel Bhavin N; Shrivastav Pranav S^*; Sanyal Mallika

来源：Bioanalysis, 2011, 3(19): 2177-2194.

DOI：10.4155/BIO.11.226

A simple, selective and sensitive LC-MS/MS assay has been developed for the determination of minocycline in human plasma. Plasma samples were prepared by protein precipitation, followed by chromatographic separation on a HyPURITY (TM) C8 (100 mm x 4.6 mm, 5 mu m) column under isocratic conditions. The LOD and LLOQ of the method were 0.50 and 20.0 ng/ml, respectively. The intra-batch and inter-batch precision (% coefficient of variation) across quality control levels was less than 8.0%. For a set of incurred samples the percentage change in concentration was within +/- 9.0%. The method was successfully applied to a bioequivalence study of 135 mg minocycline tablet formulation in 14 healthy Indian males. The reproducibility in the measurement of study data was demonstrated by incurred sample reanalysis.

• 出版日期2011-10