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摘要
This work developed a novel process for erythromycin (EM) separation from fermentation broth, in which fixed-bed adsorption onto macroporous resins and aqueous crystallization were employed, aiming at efficient recovery of EM and effective removal of erythromycin C (EC) which is an analogue of desired erythromycin A (EA). Breakthrough curves of fixed-bed adsorption of EM were investigated, and the experimental data were correlated with Adams-Bohart model. The results showed that pore diffusion was the rate-limiting step of the adsorption, and the adsorption rate constant would decrease as inlet EM concentration increases. Through adsorption and subsequent desorption by butyl acetate (BA), more than 96% of EM could be recovered from filtrated fermentation broth into BA elution, where EM concentration was more than 65 mg/ml. EM in the BA elution was then transferred into KH2PO4-K2HPO4 buffer solution (pH 7.0) through evaporating azeotrope of BA and water. An aqueous crystallization in this buffer solution was then performed to remove EC. The effects of pH and temperature on this crystallization were studied. The results showed that, with the increase of pH from 9.0 to 10.0 or temperature from 35 degrees C to 55 degrees C, the crystallization yield of EA increased while that of EC increased first and then decreased. At pH 9.8 and temperature 50 degrees C, crystallization yields of EA and EC were 92.3% and 41.9%, respectively, thus the ratio of EA to EC in the product was increased 2.2 times.
- 出版日期2013-9-15
- 单位华东理工大学
