Down-regulation of Lsm1 is involved in human prostate cancer progression

作者：Takahashi S^*; Suzuki S; Inaguma S; Cho YM; Ikeda Y; Hayashi N; Inoue T; Sugimura Y; Nishiyama N; Fujita T; Ushijima T; Shirai T

来源：British Journal of Cancer, 2002, 86(6): 940-946.

DOI：10.1038/sj/bjc/6600163

• Lsm1
• prostate cancer
• tumour progression

Elucidation of genetic alterations is an approach to understanding the under-lying molecular mechanisms of progression of human prostate cancers. We have searched for genes differentially expressed in advanced prostate cancers using cDNA-representational difference analysis, and thereby isolated the Lsm I as one of down-regulated gene. An Lsm I expression vector was transfected into PC3 cells, normally featuring down-regulated Lsm 1, and four transfectants were established. No differences in morphology or cell proliferation were evident in comparison with parent PC3 or PC3/mock-transfectants, In contrast, significant suppression of invasive potential or metastatic ability of Lsm I transfectants was observed in the Matrigel chemoinvasion assay and in nude mice, respectively, With human prostate cancers, almost all of informative prostatectomised cases without neoadjuvant therapy showed allelic retention in the Lsm I region, whereas refractory cancers frequently showed allelic loss in this region. No critical gene mutations were found in open reading frame of Lsm I in prostate cancers examined by PCR-SSCP analysis, including localised and refractory cancers. These results suggest that Lsm I is deeply involved in prostate cancer progression through its down-regulation, independent of any gene mutation.

• 出版日期2002-3-18