科研之友
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摘要
A major gene controlling chlorophyll deficiency (phenotyped by yellow leaf color, yl) in sunflower was identified and mapped in an F(2) population derived from a cross between two breeding tines. Greenness degree was scored by a hand-held chlorophyll meter in the F(2) population. Leaf tissue from the parents, F, hybrids, and some F(2). progenies were also sampled to determine the chlorophyll content. Atl. F(2) plants had normal green leaf color and the segregation of the plants in the F(2) population fits the monogenic ratio (chi(2)((3:1)) = 0.03, p>0.9), indicating that leaf color is a monogenic trait with normal green dominant over yellow leaf color in this population. The contents of chlorophyll a, chlorophyll b, and total chlorophyll. in the yellow-leafed tines were reduced by 41.6%, 53.5%, and 44.3%, respectively, in comparison with those in the green-leafed tines. Genetic mapping with molecular markers positioned the gene, yl, to linkage group 10 of sunflower. An SSR marker, ORS 595, cosegregated with yl, and a TRAP marker, B26P17ga5-300, was linked to yl with a genetic distance of 4.2cM. The molecular marker tightly linked to the chlorophyll deficiency gene wilt provide insight into the process of chlorophyll metabolism in sunflower. Published by Elsevier GmbH.
- 出版日期2009
- 单位华中农业大学
