Affinity-Based Methods in Drug-Target Discovery

作者：Rylova Gabriela; Ozdian Tomas; Varanasi Lakshman; Soural Miroslav; Hlavac Jan; Holub Dusan; Dzubak Petr; Hajduch Marian^*

来源：Current Drug Targets, 2015, 16(1): 60-76.

DOI：10.2174/1389450115666141120110323

• Affinity purification
• drug-target discovery
• immobilization
• mass-spectrometry
• probe
• quantification

Target discovery using the molecular approach, as opposed to the more traditional systems approach requires the study of the cellular or biological process underlying a condition or disease. The approaches that are employed by the "bench" scientist may be genetic, genomic or proteomic and each has its rightful place in the drug-target discovery process. Affinity-based proteomic techniques currently used in drug-discovery draw upon several disciplines, synthetic chemistry, cell-biology, biochemistry and mass spectrometry. An important component of such techniques is the probe that is specifically designed to pick out a protein or set of proteins from amongst the varied thousands in a cell lysate. A second component, that is just as important, is liquid-chromatography tandem massspectrometry (LC-MS/MS). LC-MS/MS and the supporting theoretical framework has come of age and is the tool of choice for protein identification and quantification. These proteomic tools are critical to maintaining the drug-candidate supply, in the larger context of drug discovery.

• 出版日期2015