科研之友
微信
新浪微博
Facebook
分享链接
摘要
A simple. sensitive and reliable ion-pair reverse phase high-performance liquid chromatography method has been developed and validated for the simultaneous determination of five fluoroquinolone residues in milk. The studied fluoroquinolones were ofloxacin, ciprofloxacin enrofloxacin, norfloxacin and sarafloxacin. Chromatographic separation was carried out on a Venusil XBP C(18) column, in isocratic mode, with 0.025 mol/L H(3)PO(4) solution, adjusted to pH 3.2 with 13 mmol/L tetrabutylammonium bromide-acetonitrile (96:4, v/v) as mobile phase. Good linearity over the investigated concentration range was observed, with mean values of con-elation coefficients higher than 0.9993 for all the analytes studied. The limit of detection (LOD) and the limit of quantification (LOQ) with acceptable precision were 14.09, 46.97 mg/kg for ofloxacin, 4.39, 14.64 mg/kg for norfloxacin, 10.18, 33.94 mg/kg for ciprofloxacin, 10.53, 35.12 mg/kg for enrofloxacin and 17.44, 58.14 mg/kg for sarafloxacin, respectively. These values are in compliance with requirements for monitoring of maximum residues levels. Overall recoveries from spiked milk samples ranged from 80.7 to 96.92 % with relative standard deviations lower than 4.23 %.
- 出版日期2011-9
- 单位河北大学
