Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts

作者：Fusi Eleonora^*; Rebucci Raffaella; Pecorini Chiara; Campagnoli Anna; Pinotti Luciano; Saccone Francesca; Cheli Federica; Purup Stig; Sejrsen Kristen; Baldi Antonella

来源：Toxins, 2010, 2(6): 1265-1278.

DOI：10.3390/toxins2061265

• ochratoxin A
• alpha-tocopherol
• DNA damage
• fibroblasts
• swine

The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of alpha-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM alpha-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that alpha-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.

• 出版日期2010-6