Maribacter confluentis sp nov., isolated from the junction between the ocean and a freshwater spring

作者：Park Sooyeon; Jung Yong Taek; Park Ji Min; Won Sung Min; Yoon Jung Hoon^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2015, 65(9): 3079-3085.

DOI：10.1099/ijs.0.000379

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK2-2(T), was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK2-2(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 2.0-3.0 To (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain SSK2-2(T) fell within the clade comprising the type strains of species of the genus Maribacter, joining the type strain of Maribacter sedimenticola with which it shared 99.4 % similarity. Sequence similarities to the type strains of other Maribacter species were 94.6-98.2 clo. Strain SSK2-2(T) contained MK-6 as the predominant menaquinone and iso-C-17: 0 3-OH, iso-C-15 : 0, iso-C-15 : 1 G, summed feature 3 (C-16: 1 omega 7c and/or C-16 : 1 omega 6c) and summed feature 9 (iso-C-17 : 1 omega 9c and/or 10-methyl C-16 : 0) as the major fatty acids. The major polar lipids of strain SSK2-2(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-2(T) was 38.2 molo/o and mean levels of DNA DNA relatedness with the type strains of four phylogenetically related species of the genus Maribacter were 11-24 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK2-2(T) is separate from other Maribacter species. On the basis of the data presented, strain SSK2-2(T) is considered to represent a novel species of the genus Maribacter, for which the name Maribacter con fluentis sp. nov. is proposed. The type strain is SSK2-2(T) (=KCTC 42604(T)=CECT 8869(T)).

• 出版日期2015-9