A replicon trans-packaging system reveals the requirement of nonstructural proteins for the assembly of bovine viral diarrhea virus (BVDV) virion

作者：Liang Delin; Chen Limei; Ansari Israrul H; Gil Laura H V G; Topliff Christina L; Kelling Clayton L; Donis Ruben O^*

来源：Virology, 2009, 387(2): 331-340.

DOI：10.1016/j.virol.2009.02.019

• BVDV
• Pseudo-particles
• Trans-packaging
• Complementation
• NS2-3-4A precursor
• Replication
• Virion assembly

A selective trans-packaging system was developed to produce and isolate bovine viral diarrhea Virus (BVDV) pseudo-particles with complementing reporter replicons and their packaging proteins expressed in trans with recombinant vaccinia Virus. The encapsidation Of replicon rNS3-5B was dependent not only on the in trans expression of structural proteins C, E(rns), El and E2, but also the nonstructural proteins, p7 and contiguous precursor NS2-3-4A. Nonstructural p7, NS4B. NS5A or NS5B Could be expressed in cis and in trans with precursor NS2-3-4A without significantly affecting virion assembly efficiency. NS2-3-4A was identified as an in trans functional precursor in virion assembly. BVDV genomes with mutant NS5B, which did not undergo active replication, were packaged 5-fold less efficiently than the intact genomes demonstrating the importance of replication in virion packaging. These results suggest that genome replication and assembly are closely associated, consistent with a model in which these two steps are Coupled for maximum efficiency.

• 出版日期2009-5-10