Molecular engineering of industrial enzymes: recent advances and future prospects

作者：Yang, Haiquan; Li, Jianghua; Shin, Hyun-dong; Du, Guocheng; Liu, Long^*; Chen, Jian

来源：Applied Microbiology and Biotechnology, 2014, 98(1): 23-29.

DOI：10.1007/s00253-013-5370-3

• Enzymes
• Directed evolution
• Site-directed mutagenesis
• Saturation mutagenesis
• Truncation
• Fusion

Many enzymes are efficiently produced by microbes. However, the use of natural enzymes as biocatalysts has limitations such as low catalytic efficiency, low activity, and low stability, especially under industrial conditions. Many protein engineering technologies have been developed to modify natural enzymes and eliminate these limitations. Commonly used protein engineering strategies include directed evolution, site-directed mutagenesis, truncation, and terminal fusion. This review summarizes recent advances in the molecular engineering of industrial enzymes and discusses future prospects in this field. We expect this review to increase interest in and advance the molecular engineering of industrial enzymes.

• 出版日期2014-1
• 单位江南大学