Application of direct-injection detector integrated with the multi-pumping flow system to chemiluminescence determination of the total polyphenol index

作者：Nalewajko Sieliwoniuk Edyta^*; Iwanowicz Magdalena; Kalinowski Slawomir; Kojlo Anatol

来源：Analytica Chimica Acta, 2016, 911: 82-91.

DOI：10.1016/j.aca.2016.01.033

• Direct-injection detector
• Multi-pumping flow system
• Polyphenolic antioxidants
• Chemiluminescence
• Nanocolloidal manganese(IV)
• Green analytical chemistry

In this work, we present a novel chemiluminescence (CL) method based on direct-injection detector (DID) integrated with the multi-pumping flow system (MPFS) to chemiluminescence determination of the total polyphenol index. In this flow system, the sample and the reagents are injected directly into the cone-shaped detection cell placed in front of the photomultiplier Such construction of the detection chamber allows for fast measurement of the CL signal in stopped-flow conditions immediately after mixing the reagents. The proposed DID-CL-MPFS method is based on the chemiluminescence of nanocolloidal manganese(IV)-hexametaphosphate-ethanol system. The application of ethanol as a sensitizer, eliminated the use of carcinogenic formaldehyde. Under the optimized experimental conditions, the chemiluminescence intensities are proportional to the concentration of gallic acid in the range from 5 to 350 ng mL(-1). The DID-CL-MPFS method offers a number of advantages, including low limit of detection (0.80 ng mL(-1)), high precision (RSD = 3.3%) and high sample throughput (144 samples h(-1)) as well as low consumption of reagents, energy and low waste generation. The proposed method has been successfully applied to determine the total polyphenol index (expressed as gallic acid equivalent) in a variety of plant-derived food samples (wine, tea, coffee, fruit and vegetable juices, herbs, spices).

• 出版日期2016-3-10