Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice

作者：Cheng, Jianhui; Zhang, Zuofa; Zheng, Zhiguo; Lv, Guoying; Wang, Liangyan; Tian, Bing; Hua, Yuejin^*

来源：Tropical Journal of Pharmaceutical Research, 2014, 13(4): 573-580.

DOI：10.4314/tjpr.v13i4.13

• Antioxidant
• Deinoxanthin
• Deinococcus radiodurans
• Hepatoprotective
• Carbon tetrachloride
• Liver damage

Purpose: To investigate the antioxidant activity and hepatoprotective effect of deinoxanthin-rich extract from Deinococcus radiodurans (EDR) against CCl4-induced liver injury in mice. @@@ Methods: The ethanol extract of EDR was analyzed by liquid chromatography/mass spectrometry (LC/MS), and its antioxidant activity was examined using in vitro assays for reducing power, iron chelating activity and lipid peroxidation. The extract was also evaluated for its hepatoprotective activity against carbon tetrachloride-induced liver injury in mice. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) in serum, and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissue, as well as hepatic malondialdehyde (MDA) levels, were measured to monitor liver injury. Damage to liver cells was assessed by histology. @@@ Results: EDR displayed strong reducing activity and lipid peroxidation inhibition activity in vitro. Pretreatment with EDR (400 mg/kg b.w.) significantly reduced activities of serum ALT, AST and ALP, as well as hepatic MDA levels (p < 0.05), but increased the activities of GSH-Px, CAT and SOD. Histopathological assessment showed that liver tissue damage was decreased by the protective effect of EDR. @@@ Conclusion: The results show that EDR can protect mice against CCl4-induced hepatic damage by its antioxidant and free radical scavenging activities.

• 出版日期2014-4
• 单位浙江大学; 浙江省肿瘤医院; 浙江省农业科学院