科研之友
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摘要
We describe a sensitive biosensing system combining magnetic relaxation switch diagnosis and colorimetric detection of human alpha-thrombin, based on the aptamer-protein interaction induced aggregation of Fe3O4@Au nanoparticles. To demonstrate the concept, gold-coated iron oxide nanoparticle was synthesized by iterative reduction of HAuCl4 onto the dextran-coated Fe3O4 nanoparticles. The resulting core-shell structure had a flowerlike shape with pretty narrow size distribution (referred to as "nanorose"). The two aptamers corresponding to human alpha-thrombin were conjugated separately to two distinct nanorose populations. Once a solution containing human alpha-thrombin was introduced, the nanoroses switched from a well dispersed state to an aggregated one, leading to a change in the spin-spin relaxation time (T-2) as well as the UV-Vis absorption spectra of the solution. Thus the qualitative and quantitative detection method for human alpha-thrombin was established. The dual-mode detection is clearly advantageous in obtaining a more reliable result; the detection range is widened as well. By using the dual-mode detection method, a detectable T-2 change is observed with 1.0 nM human alpha-thrombin, and the detection range is from 1.6 nM to 30.4 nM.
