科研之友
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摘要
p blood group has a unique antigen: p1. it was identified in various parasite species. the aim of this work was to determine a. lumbricoides∩s inhibitory capacity in relation to p1 antigen. the work was based on 11 parasite extracts (ae). the semiquantitative inhibition agglutination technique was used to assess the extracts∩ inhibitory capacity in relation to p1 antigen. two series of anti- p1 monoclonal antibody dilutions were prepared. pbs was added to the first series and an equal volume of ae to the second. suspensions of p1 red cells were used as a revealing system. monoclonal antibody titres were determined in both series. the agglutination was considered significative when there was a difference of two or more dilutions between the titres. eight of the 11 extracts studied presented p1 epithopes. inhibitory power of these 8 extracts was calculated and it was related with their protein concentrations to compare the inhibitory capacity among the extracts. the technique used made it possible to prove p system epithopes presence and to compare the extracts∩ inhibitory capacity in relation to p1 epithopes.
- 出版日期2007
