Architecture and DNA Recognition Elements of the Fanconi Anemia FANCM-FAAP24 Complex

作者：Coulthard Rachel; Deans Andrew J; Swuec Paolo; Bowles Maureen; Costa Alessandro; West Stephen C; McDonald Neil Q^*

来源：Structure, 2013, 21(9): 1648-1658.

DOI：10.1016/j.str.2013.07.006

Fanconi anemia (FA) is a disorder associated with a failure in DNA repair. FANCM (defective in FA complementation group M) and its partner FAAP24 target other FA proteins to sites of DNA damage. FANCM-FAAP24 is related to XPF/MUS81 endonucleases but lacks endonucleolytic activity. We report a structure of an FANCM C-terminal fragment (FANCM(CTD)) bound to FAAP24 and DNA. This S-shaped structure reveals the FANCM (HhH)(2) domain is buried, whereas the FAAP24 (HhH)(2) domain engages DNA. We identify a second DNA contact and a metal center within the FANCM pseudo-nuclease domain and demonstrate that mutations in either region impair double-stranded DNA binding in vitro and FANCM-FAAP24 function in vivo. We show the FANCM translocase domain lies in proximity to FANCM(CTD) by electron microscopy and that binding fork DNA structures stimulate its ATPase activity. This suggests a tracking model for FANCM-FAAP24 until an encounter with a stalled replication fork triggers ATPase-mediated fork remodeling.

• 出版日期2013-9-3