NUCLEOTIDE-SEQUENCE OF AN ENDO-1,4-BETA-GLUCANASE GENE (CELA) FROM CLOSTRIDIUM-JOSUI

作者：FUJINO T; OHMIYA K

来源：Journal of Fermentation and Bioengineering, 1992, 73(4): 308-313.

DOI：10.1016/0922-338X(92)90189-2

The nucleotide sequence of a DNA fragment containing an endo-1,4-beta-glucanase (EG-1) gene of Clostridium josui was determined by the dideoxy-chain termination method. The EG-1 coding sequence was an open reading frame encoding 369 amino acids, and a putative promoter sequence was located in the upstream region of the open reading frame. The N-terminal amino acid sequence of the endoglucanase (EG-1C) purified from the Escherichia coli transformant (JM103/pUCJ1) was consistent with the deduced sequence from 30Val to 44Lys. The estimated molecular weights of the precursor and the mature enzymes were 41,774 and 38,352, respectively. The region of amino acids from 61st to 335th of the enzyme revealed high homology with those of Bacillus sp. and Clostridium acetobutylicum endoglucanases.

• 出版日期1992