Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor

作者：Zhu Anwei; Tian Yang^*; Liu Haiqing; Luo Yongping

来源：Biomaterials, 2009, 30(18): 3183-3188.

DOI：10.1016/j.biomaterials.2009.02.019

• Nanoporous Au film
• Direct electron transfer
• Biosensor
• Cytochrome c
• Hydrogen peroxide (H2O2)

A layer-by-layer route to prepare nanoporous Au film materials on transparent ITO substrates is reported by alternatively assembling Au and Ag nanoparticles through 1,5-pentanedithiol as a cross-linker, followed by that Ag nanoparticles are dissolved at room temperature in HAuCl4 solution. Electron transfer of cytochrome c (cyt. c) - an excellent model for investigation of biomolecules, is greatly facilitated at the nanoporous Au film with electron transfer rate constant (k(s)) of 3.9 s(-1). Meanwhile, cyt. c adsorped onto the nanoporous Au film still maintain its enzymatic activity toward H2O2. On the basis of these experimental results, the cyt. c-nanoporous Au film is exploited to an amperometric biosensor for H2O2 with high selectivity, broad linear range, low detection limit, and long stability.

• 出版日期2009-6
• 单位同济大学