Mirror-image polymerase chain reaction

作者：Jiang, Wenjun; Zhang, Baochang; Fan, Chuyao; Wang, Min; Wang, Jiaxing; Deng, Qiang; Liu, Xianyu; Chen, Ji; Zheng, Jishen; Liu, Lei^*; Zhu, Ting F.^*

来源：Cell Discovery, 2017, 3(1): 17037.

DOI：10.1038/celldisc.2017.37

• chirality
• D-amino acid
• L-DNA
• mirror-image polymerase chain reaction (miPCR)
• Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4)

The construction of mirror-image biological systems may open the next frontier for biomedical technology development and discovery. Here we have designed and chemically synthesized a mutant version of the thermostable Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) consisting of D-amino acids. With a total peptide length of 358 amino acid residues, it is the largest chemically synthesized D-amino acid protein reported to date. We show that the D-polymerase is able to amplify a 120-bp L-DNA sequence coding for the Escherichia coli 5S ribosomal RNA gene rrfB by mirror-image polymerase chain reaction, and that both the natural and mirror-image systems operate with strict chiral specificity. The development of efficient miPCR systems may lead to many practical applications, such as mirror-image systematic evolution of ligands by exponential enrichment for the selection of therapeutically promising nuclease-resistant L-nucleic acid aptamers.

• 出版日期2017-10-17
• 单位清华大学; 中国科学技术大学