UVC-priming mediated modulation of forskolin biosynthesis key genes against Macrophomina root rot of Coleus forskohlii-A tissue culture based sustainable approach

作者：Gangopadhyay Moumita^*; Gantait Saikat; Palchoudhury Shouroseni; Ali Md Nasim; Mondal Chhanda; Pal Arun Kumar

来源：Phytochemistry Letters, 2016, 17: 36-44.

DOI：10.1016/j.phytol.2016.06.007

• Callus
• Diterpene synthase (CfTPS)
• GGPP synthase
• Macrophomina phaseolina
• Ultraviolet-C

Coleus forskohlii is susceptible to root rot disease that reduces yield of root specific metabolite forskolin. In this communication, we first time reported sustainable management of Macrophomina phaseolina root rot in C. forskohlii via Ultraviolet-C (UVC)-hormesis using tissue culture. Compact calli were developed using 2,4-dichlorophenoxy acetic acid (2.0 mg l (1)) and tolerance level of UVC exposure was optimized. Calli exhibited 88% viability, 8.4% relative growth in biomass and higher absorbance in 2,3,5-triphenyl tetrazolium chloride analysis subsequent to 10 min of UVC exposure. Pathogenicity of M. phaseolina was reduced in UVC-primed calli-regenerated plants compared with un-primed ones under controlled condition. Plant height, leaf number, shoot and root biomass significantly improved in UVC-primed calli-regenerated plants over un-primed ones when challenged by M. phaseolina in glasshouse. Significant elevation of economically important forskolin via up-regulation of key forskolin biosynthesis genes GGPP synthase, CfTPS2 and CfTPS3 highlighted the potentiality of UVC-priming to boost plant's defence mechanism and protect from drastic economic loss.

• 出版日期2016-9