Determination of Small Molecular Soluble Protein Binding Copper in Tissue by Graphite Furnace Atomic Absorption Spectrometry after Ultrasonic-Dialysis

作者：Zhang Yuan; Han Ming; Lin Zhe Xuan; Li Hui; Li Wei Qiu; Lai Mei Chao; Luo Wen Hong^*

来源：Chinese Journal of Analytical Chemistry, 2013, 41(11): 1784-1785.

DOI：10.3724/SP.J.1096.2013.30238

• Copper
• Ultrasonic-dialysis
• Graphite furnace atomic absorption spectrometry

A dialysis-GFAAS method was established for the determination of small molecular soluble protein binding copper (DiaCu) in tissue. 25 kDa dialysis membrane was used, and the dialysate was collected for the copper determination and protein contents determination. The results showed that the detection limit was 0.12 mu g/L, with RSD < 5%. The recovery was 90% - 102%. The method was applied to determine the DiaCu in endotoximia SD rats induced by lipopolysaccharide (LPS), and it was found that the DiaCu concentration decreased in inflammatory tissue.

• 出版日期2013-11
• 单位汕头大学