Alterations in protein and amino acid metabolism in honeybees (Apis mellifera) fed different L-leucine diets during the larval stage

作者：Ma, Lanting; Wang, Ying; Zhang, Weixing; Wang, Hongfang; Liu, Zhenguo; Xu, Baohua^*

来源：Journal of Asia-Pacific Entomology, 2016, 19(3): 769-774.

DOI：10.1016/j.aspen.2016.07.005

• L-Leucine
• Protein synthesis
• TOR signaling pathway
• Amino acids balance
• Hemolymph

L-Leucine is an important factor for growth and development in animals. However, very little is known about the nutritional role and physiological function of L-Ieucine in honeybees. In this study, we determined the effects of L-leucine supplementation on protein synthesis and amino acid balance in honeybee larvae. First instar larvae were fed different L-leucine diets (6.45, 8.45 and 10.45 mg/g) during the entire larval stage. After the larvae egested urate crystals, we analyzed the protein and amino acid metabolism indexes in the larvae. There was little effect of L-leucine on larval body weight and body protein content, but the hemolymph protein concentrations were higher in 8.45 and 10.45 mg/g groups than 6.45 mg/g group. Compared to the 6.45 mg/g group, L-leucine significantly increased the contents of Ala, Leu, Lys and Met in 8.45 mg/g group and continued to be increased in 10.45 mg/g group. L-Leucine affected many TOR signaling pathway- and L-leucine metabolism-related genes, including tor, 4Ebp1, bcaat et al. Moreover, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creative kinase (CK) and lactate dehydrogenase (LDH) activities as well as the triglycerides (TG), total cholesterol (TCHO), uric acid (UA) and urea nitrogen (URER) contents in haemolymph were significantly different among treatments. These results suggested that many factors involved in the metabolism of L-leucine and larval hemolymph play important roles in honeybee protein synthesis and amino add balance.

• 出版日期2016-9
• 单位山东农业大学