Methods for Quantification of Soil-Transmitted Helminths in Environmental Media: Current Techniques and Recent Advances

作者：Collender Philip A; Kirby Amy E; Addiss David G; Freeman Matthew C^*; Remais Justin V

来源：Trends in Parasitology, 2015, 31(12): 625-639.

DOI：10.1016/j.pt.2015.08.007

Limiting the environmental transmission of soil-transmitted helminths (STHs), which infect 1.5 billion people worldwide, will require sensitive, reliable, and cost-effective methods to detect and quantify STHs in the environment. We review the state-of-the-art of STH quantification in soil, biosolids, water, produce, and vegetation with regard to four major methodological issues: environmental sampling; recovery of STHs from environmental matrices; quantification of recovered STHs; and viability assessment of STH ova. We conclude that methods for sampling and recovering STHs require substantial advances to provide reliable measurements for STH control. Recent innovations in the use of automated image identification and developments in molecular genetic assays offer considerable promise for improving quantification and viability assessment.

• 出版日期2015-12