Downregulation of LIMK1-ADF/cofilin by DADS inhibits the migration and invasion of colon cancer

作者：Su, Jian; Zhou, Yujuan; Pan, Zhibing; Shi, Ling; Yang, Jing; Liao, Aijun; Liao, Qianjin^*; Su, Qi^*

来源：Scientific Reports, 2017, 7(1): 45624.

DOI：10.1038/srep45624

• HUMAN BREAST-CANCER
• CELL LUNG-CANCER
• DIALLYL DISULFIDE
• MESENCHYMAL TRANSITION
• ACTIN CYTOSKELETON
• POOR-PROGNOSIS
• LIM-KINASE
• EXPRESSION
• GROWTH
• PHOSPHORYLATION

This study aimed to explore whether the downregulation of LIM kinase 1 (LIMK1)-actin depolymerization factor (ADF, also known as destrin)/cofilin by diallyl disulfide (DADS) inhibited the migration and invasion of colon cancer. Previous studies have shown that silencing LIMK1 could significantly enhance the inhibitory effect of DADS on colon cancer cell migration and invasion, suggesting that LIMK1 was a target molecule of DADS, which needed further confirmation. This study reported that LIMK1 and destrin were highly expressed in colon cancer and associated with poor prognosis of patients with colon cancer. Also, the expression of LIMK1 was positively correlated with the expression of destrin. The overexpression of LIMK1 significantly promoted colon cancer cell migration and invasion. DADS obviously inhibited migration and invasion by suppressing the phosphorylation of ADF/cofilin via downregulation of LIMK1 in colon cancer cells. Furthermore, DADS-induced suppression of cell proliferation was enhanced and antagonized by the knockdown and overexpression of LIMK1 in vitro and in vivo, respectively. Similar results were observed for DADS-induced changes in the expression of vimentin, CD34, Ki-67, and E-cadherin in xenografted tumors. These results indicated that LIMK1 was a potential target molecule for the inhibitory effect of DADS on colon cancer cell migration and invasion.

• 出版日期2017-3-30
• 单位湖南省肿瘤医院; 中南大学; 南华大学